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The Effect Of Chemical Treatment On Oil Sand End-pit-lake Microbial Communities: An Investigation For A Proxy Of Reclamation Status.

Date

2017

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Abstract

Genetic analysis of microbial communities residing in Syncrude’s Base Mine Lake was performed before and after chemical treatment of the surface water cap with liquid aluminium sulfate (alum) solution. 16S and 18S rRNA genes were amplified with pfu polymerase chain reaction, purified, and barcoded with Illumina primers for reading sequences. Bioinformatic analysis with QIIME software demonstrated that alum addition resulted in a significant community composition shift among eukaryotes in pre/post alum addition timeframes. Prokaryotic communities were relatively less impacted but showed some variation among target taxonomic clades. A discussion of the microbiological community responses to environmental manipulation provides insight into the benefits and shortcomings of end-pit lake technology. The results from this investigation provide a positive indication that Base Mine Lake is headed in the correct direction to achieve reclamation status. Findings can be applied to optimize future end-pit lake endeavours.

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Rawluk, S. (2017). The Effect Of Chemical Treatment On Oil Sand End-pit-lake Microbial Communities: An Investigation For A Proxy Of Reclamation Status. (Unpublished report). University of Calgary, Calgary, AB.

URI

http://hdl.handle.net/1880/109749

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