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Structural organization of DNA–protein complexes of chromatin studied by vibrational and electronic circular dichroism

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dc.contributor.authorPolyanichko, Alexander
dc.contributor.authorWieser, Helmut
dc.date.accessioned2018-09-27T12:03:37Z
dc.date.available2018-09-27T12:03:37Z
dc.date.issued2010-01-01
dc.date.updated2018-09-27T12:03:37Z
dc.description.abstractStructure and functioning of chromatin is determined by interactions of DNA with numerous nuclear proteins. The most abundant and yet not completely understood non-histone chromosomal proteins are those belonging to a High Mobility Group (HMG) namely HMGB1. The interplay of this protein on DNA with linker histone H1 and other proteins determines both structure and functioning of the chromatin. A combination of UV and IR absorption and circular dichroism (CD) spectroscopy was applied to investigate the structure and formation of large supramolecular DNA–protein complexes. This combination of techniques was used to overcome limitations of UV-CD (ECD) spectroscopy due to considerable light scattering in such solutions. Based on the analysis of FTIR and UV circular dichroism spectra and AFM imaging the interaction of DNA with high-mobility group non-histone chromatin protein HMGB1 and linker histone H1 was studied.
dc.description.versionPeer Reviewed
dc.identifier.citationAlexander Polyanichko and Helmut Wieser, “Structural organization of DNA–protein complexes of chromatin studied by vibrational and electronic circular dichroism,” Spectroscopy, vol. 24, no. 3-4, pp. 239-244, 2010. doi:10.3233/SPE-2010-0472
dc.identifier.doihttps://doi.org/10.3233/SPE-2010-0472
dc.identifier.urihttp://hdl.handle.net/1880/108453
dc.identifier.urihttps://doi.org/10.11575/PRISM/45418
dc.language.rfc3066en
dc.rights.holderCopyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleStructural organization of DNA–protein complexes of chromatin studied by vibrational and electronic circular dichroism
dc.typeJournal Article

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